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Linhan Sun

Plant Biology Grad Student at Penn State.

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Week 1

3.9-3.16 文献总结

SI and UI(Unilateral Incompatibility) in Solanum

Read:

Li W, Chetelat R T. A pollen factor linking inter-and intraspecific pollen rejection in tomato[J]. Science, 2010, 330(6012): 1827-1830.

  • SI*SC rule of UI

  • QTL ui6.1=WD40+Cullin1(CUL1)

  • Skp1-Cullin1-F box complex/SLF-SBP-CUL1 complex

  • all green fruit species(SI or SC) have ‘shorter’ mRNA of CUL1

  • red/orange fruit species(all SC) have ‘longer’ mRNA (436bp deletion)

  • UI may be regulated by a similar mechanism as SNare-dependent SI

RACE

Tovar‐Méndez A, Kumar A, Kondo K, et al. Restoring pistil‐side self‐incompatibility factors recapitulates an interspecific reproductive barrier between tomato species[J]. The Plant Journal, 2014, 77(5): 727-736.

  • Introducing functional S-RNase/HT-A/HT-B of SI species into cultivated tomato

  • S-RNase+HT-A/B is sufficient for recapitulate SI (redundant mechanism)

  • mating system transition from SI to SC

  • transition would affect interspecific compatibility

AP1 regulated determinate growth of sepal axil cells via cytokinin

Read:

Han Y, Zhang C, Yang H, et al. Cytokinin pathway mediates APETALA1 function in the establishment of determinate floral meristems in Arabidopsis[J]. Proceedings of the National Academy of Sciences, 2014, 111(18): 6840-6845.

transient transfection assay

glucocoriticoid receptor (GR) fusion

ABP1 is not essential

Read:

Gao Y, Zhang Y, Zhang D, et al. Auxin binding protein 1 (ABP1) is not required for either auxin signaling or Arabidopsis development[J]. Proceedings of the National Academy of Sciences, 2015, 112(7): 2275-2280.

Cellular immunization generated knockdown alleles(antibody mutants)

TILLING mutant

Commentary:

Tena G. Auxin signalling: ABP1 springs a surprise[J]. Nature Plants, 2015, 1(3).

Liu C M. Auxin Binding Protein 1 (ABP1): A matter of fact[J]. Journal of integrative plant biology, 2015.

Haploid Induction via Centromere-Mediated Genome Elimination

(First report)

Ravi M, Chan S W L. Haploid plants produced by centromere-mediated genome elimination[J]. Nature, 2010, 464(7288): 615-618.

(mechanism)

Ravi M, Shibata F, Ramahi J S, et al. Meiosis-specific loading of the centromere-specific histone CENH3 in Arabidopsis thaliana [J]. PLoS genetics, 2011, 7(6): e1002121.

(review)

Comai L. Genome elimination: translating basic research into a future tool for plant breeding[J]. PLoS biology, 2014, 12(6): e1001876.

(DH line of Arabidopsis)

Seymour D K, Filiault D L, Henry I M, et al. Rapid creation of Arabidopsis doubled haploid lines for quantitative trait locus mapping[J]. Proceedings of the National Academy of Sciences, 2012, 109(11): 4227-4232.

(Apoximis Fixation)

Marimuthu M P A, Jolivet S, Ravi M, et al. Synthetic clonal reproduction through seeds[J]. Science, 2011, 331(6019): 876-876.

(Review of RNA regulated centromere)

Gent J I, Dawe R K. RNA as a structural and regulatory component of the centromere[J]. Annual review of genetics, 2012, 46: 443-453.

(maize)

Zhao X, Xu X, Xie H, et al. Fertilization and uniparental chromosome elimination during crosses with maize haploid inducers[J]. Plant physiology, 2013, 163(2): 721-731.

Apoximis of crop plants

Marimuthu M P A, Jolivet S, Ravi M, et al. Synthetic clonal reproduction through seeds[J]. Science, 2011, 331(6019): 876-876.

Singh M, Goel S, Meeley R B, et al. Production of viable gametes without meiosis in maize deficient for an ARGONAUTE protein[J]. The Plant Cell Online, 2011, 23(2): 443-458.

(MiME) d’Erfurth I, Jolivet S, Froger N, et al. Turning meiosis into mitosis[J]. PLoS biology, 2009, 7(6): e1000124.

全世界科学家利用单性生殖这种强大工具创建能生产维持活力的后代种子、繁衍与母本相同后代的杂交品种。单性生殖在400多个植物物种自然发生，但是在一些重要作物，如水稻、小麦和玉米中，却是罕见的。

法国国家科学研究中心(CNRS)、法国国立农业研究所（INRA）、奥地利分子病理学研究所的科学家们在开发单性生殖作物方面取得重要突破。该小组解决了单性生殖研究的一个重要障碍：减数分裂。减数分裂是一种细胞分裂方式，产生混合父母性状的雌雄配子。通过模式植物拟南芥三个基因突变的结合，研究小组建立了一个称为“MiMe”的基因型，其减数分裂完全被有丝分裂和无性细胞分裂取代。

Rapha.l Mercier及其同事针对生殖细胞分裂独有的三个进程：

.利用Atspo11 - 1基因突变重组或配对染色体

.利用Atrec8基因突变隔离或分离染色体

.在细胞分裂第二轮利用osd1基因突变

然而，实现单性生殖依然是一个遥远目标。专家预测，单性生殖作物仍然可能需要15年才能进入市场。有丝分裂取代减数分裂的缺点是，每一代染色体数目都会增加，染色体数目上升可导致生育率下降。科学家们还必须找到一种孤雌生殖产生可育种子的方法。尽管如此，全世界科学家都承认Mercie及其同事的发现极具意义。

(Review)

Barcaccia G, Albertini E. Apomixis in plant reproduction: a novel perspective on an old dilemma[J]. Plant reproduction, 2013, 26(3): 159-179.

A rotating view of auxin signaling

(Lecture: Dr.Jianru Zuo, unpublished work)

  • root development of rice

  • lrt2(lateral rootless 2) mutant

  • LRT2 encodes a cyclophilin, a PPIase (Pro cis-trans isomerase). Substrate?

  • osiaa mutant phenocopies lrt2

  • T105C106 in IAA protein is transformed to CC catalyzed by LRT2, which subsequently promotes interaction of IAAx with TIR.

  • Auxin functions as ‘molecular glue’

Related:

Li M, Ma X, Chiang Y H, et al. Proline isomerization of the immune receptor-interacting protein RIN4 by a cyclophilin inhibits effector-triggered immunity in Arabidopsis[J]. Cell host & microbe, 2014, 16(4): 473-483.

Rice Chilling Tolerance(COLD1)

Ma Y, Dai X, Xu Y, et al.COLD1 confers chilling tolerance in rice[J]. Cell, 2015.

Questions:

  • How is cold sensing and cold tolerance related?

  • What’s the function of QTLs cold2-5? How would we explain their genetic contribution to chilling tolerance phenotype?

  • GWAS to detect possible SNPs?

Regulatory between RdDM and demethylation in Arabidopsis

Lei M, Zhang H, Julian R, et al. Regulatory link between DNA methylation and active demethylation in Arabidopsis[J]. Proceedings of the National Academy of Sciences, 2015: 201502279.